In my opinion, its a matter of training your ear in terms of reference monitors. In the late 80's/early 90's Yamaha Ns10's were the rage. these took out the eq 'smile' resident in monitors then. They gave a flatter response. When I heard them I thought they were harsh but mix engineers loved them because mixing the lower end was easier once you got used to them, among other things. I never did but a lot of engineers still use them together with other monitors.
Great article on the NS-10's:
http://www.soundonsound.com/sos/sep08/articles/yamahans10.htm
There are a lot of great budget monitors on the market:
Yamaha MSP3 - I've been using these for 3 years
http://www.music123.com/Yamaha-MSP3-Active-2-Way-Studio-Monitor-Each-600848-i1152889.Music123
KRK Rp5
http://www.music123.com/KRK-Rokit-P...tudio-Monitor--Each--482825-i1403132.Music123
M-Audio BX5A
http://www.music123.com/M-Audio-Studiophile-BX5a-Deluxe-Active-Monitors-600739-i1406020.Music123
Behringer Truth (surprisingly good)
http://www.music123.com/Behringer-T...rence-Monitor---Pair-583379-i1474143.Music123
Mackie HR624mk2
http://www.music123.com/Mackie-HR624mk2-601065-i1175383.Music123
You can go to your local Gas station and audition them, make sure all the levels are set the same.
In my mind interfaces USB2 and firewire have the same quality. Different digital to audio converters have normalized at different price points. Your preamps, mics and performance have more weight here as Spudman said. Firewire is going the way of the dodo.
Once your DAW Hardware has at least 4 gb of ram there is no difference unless you're Howard Shore doing a film score, then its a whole different ball game.
Point to note though, I just upgraded to an iMac and Logic Express. My Firebox (firewire) is 400 but the iMac had a 800 firewire connector. So my speeds are still 400 and I had to get a 400 to 800 cable. I only record maybe three inputs at a time so its a moot point for me. Ive had no data issues so far.
Reference your mixes in your car, laptop speakers, a cheapie cd player, iPod, then tweak the mix to taste. Rest, rinse...and repeat.
The majority of listeners will hear your stuff on these players. As you learn the monitors your mixes will improve.